Research Article

Postcatalytic spliceosome structure reveals mechanism of 3′–splice site selection

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Science  08 Dec 2017:
Vol. 358, Issue 6368, pp. 1283-1288
DOI: 10.1126/science.aar3729

Understanding splicing from the 3′ end

The spliceosome removes introns from eukaryotic mRNA precursors and yields mature transcripts by joining exons. Despite decades of functional studies and recent progress in understanding the spliceosome structure, the mechanism by which the 3′ splice site (SS) is recognized by the spliceosome has remained unclear. Liu et al. and Wilkinson et al. report the high-resolution cryo-electron microscopy structures of the yeast postcatalytic spliceosome. The structures reveal that the 3′SS is recognized through non-Watson-Crick base pairing with the 5′SS and the branch point, stabilized by the intron region and protein factors.

Science, this issue p. 1278, p. 1283