Biotechnology

Editing genomes without breaks

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Science  15 Dec 2017:
Vol. 358, Issue 6369, pp. 1398-1399
DOI: 10.1126/science.358.6369.1398-c

By introducing DNA doublestrand breaks (DSBs), most genome-editing technologies initiate endogenous DNA repair mechanisms that modify the sequences at target sites. DSBs are often toxic, and their repair is usually inefficient, thereby limiting the accuracy and scalability of these technologies. Bypassing DSBs completely, Barbieri et al. developed a new editing platform in budding yeast. DNA oligos targeting the replication fork directly replaced the genetic sequence of the region of interest after the completion of DNA replication. This technology can be easily multiplexed and can achieve single–base pair precision.

Cell 10.1016/j.cell.2017.10.034 (2017)

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