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Science  16 Feb 2018:
Vol. 359, Issue 6377, pp. 832
DOI: 10.1126/science.359.6377.832

Flow Chemistry Systems

Available in a choice of configurations, the Uniqsis FlowLab range of flow chemistry systems are affordable and easy-to-use, ideal for research and education, and the perfect option for those wishing to try flow chemistry without the associated costs of a fully automatic system. The platform uses individual reactor modules for maximum choice and flexibility. Depending on the temperature range required, choose from two configurations: FlowLab, which includes a Uniqsis HotCoil heated reactor station that delivers temperatures from ambient up to 300°C; and FlowLab Cold, which includes the Polar Bear Plus Flow and operates from –40°C to 150°C—all without the need for dry ice or liquid nitrogen. The standard system comes complete with two high-pressure pumps and dedicated FlowLab control software that enables full manual control and the ability to program a single automated experiment. But it can easily be upgraded, because the FlowLab software can autodetect and control up to three pumps and two reactor modules.

CRISPR/Cas9 Monoclonal Antibody

EpiGentek offers a mouse monoclonal antibody (mAb) raised against CRISPR/Cas9, clone 7A9, generated with a synthesized peptide corresponding to the sequence of Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1. This Anti-Cas9 mAb can detect CRISPR/Cas9 expression in target cells by Western blot, immunofluorescence, immunoprecipitation, or ELISA to confirm and verify whether guide RNA (gRNA) and Cas9 vectors are successfully transfected. Found to work in bacteria as a defense mechanism against foreign DNA, CRISPR and Cas9 (CRISPR-associated system or CRISPR-associated protein-9 nuclease) have proven to be invaluable tools to target and modify genetic sequences in gene-editing and genome-engineering applications. The CRISPR/Cas9 system allows for sequence-specific cleavage of a targeted genomic locus by delivering the RNA-guided nuclease (Cas9) and appropriate gRNAs into a cell.

Cell Imaging Systems

From cell culture to complex protein analysis to multichannel fluorescence imaging, EVOS cell imaging systems help you perform a variety of routine and specialty applications. Our proprietary LED light cube technology minimizes photobleaching, offers >50,000 hours of LED illumination, and permits precise intensity adjustments—with no darkroom and no consumables costs. The EVOS platform is convenient—with a small footprint that fits right in your cell culture room or hood. Just plug it in, turn it on, and focus on your research. EVOS captures publication-ready images in minutes.

Microplate Dispenser

The modular MultiFlo FX Microplate Dispenser replaces up to five separate liquid handlers to save time and instrument costs when dispensing into 6- to 1,536-well microplates. Up to four independent reagents are administered using Parallel Dispense technology, which combines syringe pumps and peristaltic pumps to eliminate potential reagent carryover, while optional RAD technology delivers random access dispensing (RAD) to individual wells. An optional wash module expands application versatility. The user-friendly, color touchscreen interface offers intuitive protocol creation and implementation. This compact, versatile unit enables a wide variety of uses for benchtop and integrated automation workflows, such as bulk reagent dispensing, cell seeding, cell washing/media exchange, ELISA automation, and bead washing.

Transfection Reagents

DharmaFECT transfection reagents provide efficient, reliable transfection at low siRNA/miRNA or plasmid-reagent concentrations with minimal cellular toxicity. To ensure effective siRNA or miRNA transfection, we offer DharmaFECT transfection reagents 1–4. DharmaFECT 1 is our most all-purpose transfection reagent, demonstrating efficient, low-toxicity delivery to over 80% of validated cell types. DharmaFECT 2, 3, and 4 offer distinct formulations to support a wider range of cell types, and permit more thorough optimization of transfection for high-value experiments and screening projects. DharmaFECT kb is optimized to deliver plasmid DNA at low concentrations with a minimal amount of transfection reagent and high cell viability. DharmaFECT Duo is the only optimized reagent to deliver both siRNA and miRNA reagents, along with plasmid DNA.


3C-Express is a recombinant, restriction grade, HRV3C (human rhinovirus 3C) protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro, commonly referred to as the "PreScission Site," and cleaves between Gln and Gly. It performs identically to PreScission Protease. 3C-Express has robust activity at 4°C and high specific activity—one unit of 3C-Express cleaves >95% of 100 mg of target protein at 4°C for 16 h. 3C-Express is a 47-kDa protein with both glutathione S-transferase (GST) and polyhistidine (His) tags for easy removal using either Amintra NiHIS or glutathione purification resins that recognize the cleavage site of Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro. 

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  • Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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