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Science  30 Mar 2018:
Vol. 359, Issue 6383, pp. 1549
DOI: 10.1126/science.359.6383.1549-a

35-mm Imaging Dish

ibidi's µ-Dish 35-mm Quad is a four-compartment cell culture dish that guarantees brilliant optical quality. The subdivisions enable up to four parallel, individual experiments in one dish, where applications such as transfection, immunofluorescence staining, and live-cell imaging can be conveniently performed. Quad is the ideal solution for scientists who conduct simultaneous, multiplex analysis of different cell lines or apply distinct experimental conditions. The subdivisions not only save time, but also decrease experimental costs by reducing the cell numbers and reagents needed. Its unique ibidi Polymer Coverslip Bottom guarantees superior optics for high-end microscopy. Excellent phase contrast is provided by the centered plate construction. This Ph+ (Phase Contrast +) feature avoids any meniscus effect and facilitates homogenous cell growth. 

Apoptosis/Necrosis Detection Kit

Enzo Life Science's GFP-CERTIFIED Apoptosis/Necrosis Detection Kit detects four distinct cell states: viable cells, early apoptotic cells, late apoptotic cells, and necrotic cells. Plasma membrane integrity and the display of phosphatidylserine on the plasma membrane's extracellular face are both hallmarks of apoptosis, and are used in this kit to distinguish apoptosis from necrosis. The kit features true multiplexing capabilities with green fluorescent protein (GFP) and other green fluorescent probes, and is optimized for both fluorescence microscopy and flow cytometry applications. It is validated for use with live or postfixed cells in conjunction with probes, and identifies each stage with high specificity, sensitivity, and convenience. The transition from apoptosis to necrosis is a loosely defined continuum that is crucial for understanding the development, homeostasis, and pathogenesis of different diseases; it is necessary to identify the various stages of this process to truly understand the role it plays in fields such as cancer and neuroscience research, drug discovery, and more. 

Spinning Disk Confocal Superresolution Microscope

With resolving power that surpasses the limits of conventional optical microscopes, the Olympus IXplore SpinSR10 imaging system balances speed, resolution, and efficiency in a single, flexible platform. A high frame rate and 120-nm XY resolution enable researchers to observe the fine details and workings of internal cellular structures while offering the ability to switch between superresolution, confocal, and widefield imaging modes. The SpinSR10 can quickly image as deep as 100 μm into regions that can be hard to access using traditional superresolution methods. A suite of features helps minimize phototoxicity and photobleaching when capturing 3D images, prolonging the viability of live cells during time-lapse imaging. Researchers get these benefits without having to change their existing fluorescence-labeling protocols—so live-cell, superresolution images can be captured using conventional fluorescent dyes. 

Plate Reader

The EnSight Multimode Plate Reader is a benchtop system that offers well-imaging along with label-free and labeled detection technologies. Its imaging module and easy-to-use Kaleido 2.0 data acquisition and analysis software allow you to image a 384-well plate in less than 5 min., a two-color image in around 6 min., and a three-color image in around 7 min. With its image cytometry capabilities, you can quickly and easily generate per-cell data, whether you're using fixed or live cells, performing end-point assays, or taking kinetic measurements over time—opening up a broad range of cell-based applications. The system's speed makes it ideal for assay optimization and for assessing cell-based assay quality to reveal cell-seeding errors, improper liquid handling, and bacterial contamination. You can use ready-made, click-and-go protocols or build your own from the toolbox, and the plate stacker gives you the convenience of walkaway operation. 

Live-Cell Incubator Imager

Etaluma's high-resolution, versatile, and compact inverted LS Microscopes (Lumascopes) actually fit inside your incubator, giving you stable temperatures and CO2 levels throughout your assays. They perform three-color fluorescence, phase-contrast, and brightfield microscopy, and can accommodate 1.25–100X objectives. Choose from an automated XY stage with autofocus in Z (LS720) or a manual stage (LS620) and get images, time-lapse series, and videos recorded directly to your computer. These fully functioning microscopes empower users to visualize cells from microplates, dishes, flasks, slides, microfluidic chips, or custom labware. Their quality is comparable to that of traditional, high-cost microscopes—you can finally get the big picture without the big-ticket price.

Digital Imaging System

The CELENA S is a small, powerful digital imaging system that simplifies imaging and data analysis. Integrating advanced precision optics, a highly sensitive scientific-grade complementary metal oxide semiconductor (CMOS) camera, and a computer with user-friendly software, it allows researchers to capture vivid, publication-quality images with ease. Interchangeable objectives and filter cubes accommodate a wide range of imaging needs. Researchers can use the CELENA S for multiple applications, such as capturing and analyzing multicolor fluorescence images, live-cell imaging, and automated cell counting. The new onstage incubation system features an environmental chamber, temperature controller, and gas mixer. Researchers can control temperature, humidity, and gas content with precision. Live cells can be monitored with the time-lapse function or the growth monitor.

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