Research Article

Rewritable multi-event analog recording in bacterial and mammalian cells

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Science  13 Apr 2018:
Vol. 360, Issue 6385, eaap8992
DOI: 10.1126/science.aap8992

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Writing a cell's history in its DNA

Recording cellular events could advance our understanding of cellular history and responses to stimuli. The construction of intracellular memory devices, however, is challenging. Tang and Liu used Cas9 nucleases and base editors to record amplitude, duration, and order of stimuli as stable changes in both genomic and extrachromosomal DNA content (see the Perspective by Ho and Bennett). The recording of multiple stimuli—including exposure to antibiotics, nutrients, viruses, and light, as well as Wnt signaling—was achieved in living bacterial and human cells. Recorded memories could be erased and re-recorded over multiple cycles.

Science, this issue p. eaap8992; see also p. 150