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Science  20 Apr 2018:
Vol. 360, Issue 6386, pp. 342
DOI: 10.1126/science.360.6386.342

Tissue Imaging System

The Vectra Polaris Automated Quantitative Pathology Imaging System integrates both multispectral imaging and automated slide scanning to better visualize, analyze, quantify, and phenotype immune cells in situ in FFPE tissue sections and tissue microarrays (TMAs). The fully automated system provides researchers unparalleled speed, performance, and versatility for extracting proteomic and morphometric information from intact FFPE tissue sections and TMAs. Vectra Polaris is the only instrument on the market that goes beyond basic functions such as whole-slide, brightfield, and fluorescence imaging, integrating the power of multispectral imaging in a simplified, elegant workflow. Coupled with the proven power of inForm image analysis software, its multiplexing capability enables identification and quantification of multiple biomarkers and reveals spatial context within a digital workflow, to assist researchers with better, faster decisions.

Microscope Camera

The Lumenera INFINITY3-6UR is an ideal general-purpose camera for most microscopy applications due to its 6-MP resolution, excellent color reproduction, and speed, with the light sensitivity needed for low-light applications. Built on Sony's EXview HAD II sensor technology, this camera offers extremely high dynamic range, 4.54 µm x 4.54 µm pixels, and very low noise. With its 2x2 binning, there is a fourfold increase in sensitivity along with 1.5-MP (1376x1096) resolution. INFINITY3-6UR is designed for use in a variety of scientific, life science, clinical, and industrial applications requiring optimal color reproduction, extreme sensitivity, increased resolution, and high speed.

Non-Browning Zoom Lens

Resolve Optics' Model 357 non-browning zoom lens provides high-definition-quality images over an unrivaled 10X zoom range. Featuring specialist non-browning glasses, it produces clear, sharp images without the strong yellow tint that has traditionally been a limiting issue when using radiation-resistant lenses on color sensors. It is designed to operate in environments subject to high-level radiation, such as nuclear fuel handling sites, reactor active zones, and nuclear waste storage plants. Operating at f/2, Model 357 offers high image resolution and minimum geometric distortion from 400 nm to 770 nm, and can image objects from 800 mm to infinity without add-on adapters. 

Enhanced Confocal Detector

The ZEISS Airyscan is an area detector. Unlike traditional confocal microscopes, which reject photons from outside of the focal plane at the pinhole, Airyscan detects all precious fluorescence emission photons of 1.25 a.u. Their information is then used to deliver higher sensitivity, superresolution, and high acquisition speeds. The new 2D Superresolution mode takes advantage of the fact that Airyscan captures information from the x, y, and z planes with the confocal point spread function. A new exclusive processing algorithm uses this inherent spatial information captured in a single image, specifically distinguishing between photons originating from the focal plane of 0.2 a.u. and photons from outside of this focal plane. In a traditional confocal microscope, a researcher could only close the pinhole to 0.2 a.u. to attempt the same optical sectioning. This would mean sacrificing many photons, even from the focal plane, thus reducing signal-to-noise drastically. 

Biomolecular Imager

The Amersham Typhoon NIR Plus is a modular, upgradeable near-infrared (NIR) imaging system from GE Healthcare Life Sciences. Its three-laser, flexible configuration provides two essential NIR channels as well as a third visible laser channel for triplex protein detection, to facilitate normalization and the reduction of blot-to-blot variation. Ideal for multiuser environments, it delivers extremely sensitive detection, high image resolution, and a broad linear dynamic range with multifluorescent and stained samples. Able to scan up to nine microtiter plates simultaneously, it is also suitable for high-throughput screening. NIR Plus is an affordable, user-friendly, open imaging platform compatible with most commercially available NIR detection reagents. With high (10-μm) resolution, a dynamic range greater than 5 orders of magnitude, and sensitivity as low as 3 picograms, it accommodates a wide range of samples, including tissue sections, plant leaves, and cell colonies, in addition to traditional gels, membranes, multiwell plates, and dishes.

Biological Microscopes

The CX33 and CX43 microscopes enable users to remain comfortable during long periods of routine microscopy observations. In both instruments, the stage is 70 mm lower than in the previous model, resulting in a large workspace under the eyepiece that facilitates checking or swapping samples with just one hand. In addition, the stage and coaxial focus knobs have been lowered, so users can reach them while their forearms remain on the desk. The low-positioned revolving nosepiece with a finger rest accommodates up to five objectives, enabling users to quickly change magnification with minimal arm movement. To improve durability and reduce running costs, we have added a centering-free LED light source with a long 60,000-h lifetime, providing uniform illumination and constant color temperature at any brightness level. This means users get natural color representation from a wide range of stains and can perform analyses faster and more confidently. 

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