Reusable protein-based sensors

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Science  04 May 2018:
Vol. 360, Issue 6388, pp. 504-505
DOI: 10.1126/science.360.6388.504-g

Targeted sensors for ions, molecules, proteins, or DNA can be made by immobilizing specific biomolecules onto a surface. However, it can be difficult to control the surface patterning or reversibly deposit the target molecules, which would allow for reusable surfaces. Ananth et al. selectively immobilized proteins onto silica-covered flat or porous surfaces, with control over orientation and surface density, by incorporating polyhistidine tags into the target proteins. The anchoring is reversible, as demonstrated by real-time switching. For devices that contain both gold and silica surfaces, the authors show that they can passivate the gold by using thiol chemistry while selectively coating the silica with the desired target molecules.

Small 10.1002/smll.201703357 (2018).

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