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A method for single-neuron chronic recording from the retina in awake mice

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Science  29 Jun 2018:
Vol. 360, Issue 6396, pp. 1447-1451
DOI: 10.1126/science.aas9160

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Eye can see neural activity

Organisms take up a tremendous amount of information through the visual system, which is then processed by the neural circuitry. Hong et al. developed a mesh electronics implant that is delivered by injection into mice retinas. With these devices, it is possible to obtain recordings from retinal ganglion cells over long time periods in awake, active mice. Both orientation- and direction-selective retinal ganglion cells can be monitored, as can the circadian modulation of retinal ganglion cell activity.

Science, this issue p. 1447

Abstract

The retina, which processes visual information and sends it to the brain, is an excellent model for studying neural circuitry. It has been probed extensively ex vivo but has been refractory to chronic in vivo electrophysiology. We report a nonsurgical method to achieve chronically stable in vivo recordings from single retinal ganglion cells (RGCs) in awake mice. We developed a noncoaxial intravitreal injection scheme in which injected mesh electronics unrolls inside the eye and conformally coats the highly curved retina without compromising normal eye functions. The method allows 16-channel recordings from multiple types of RGCs with stable responses to visual stimuli for at least 2 weeks, and reveals circadian rhythms in RGC responses over multiple day/night cycles.

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