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Science  03 Aug 2018:
Vol. 361, Issue 6401, pp. 518
DOI: 10.1126/science.361.6401.518

Low-Lactate Culture Media for IVF


Irvine Scientific’s Continuous Single Culture-NX is a next-generation, single-step culture medium for embryos. This formulation contains the lowest optimal concentration of lactate commercially available in culture media. Excess lactate in culture medium can contribute to stress in the embryo culture environment, affecting embryo development. Studies show that culture media with lower lactate concentrations maintain efficient metabolic rates in embryo culture for in vitro fertilization (IVF) applications. Continuous Single Culture-NX was tested for efficacy in human clinical studies, where it demonstrated an increase in blastocyst formation rates. The culture media are available in two forms: ready-to-use and presupplemented with human serum albumin, or without protein supplementation. They are designed for fertilization and embryo culture through day 5/6 of embryo development.

Validated Antibody Database

Nonspecific antibodies are a significant contributor for the current research irreproducibility problem. Antibody manufacturers and the user community are trying to address this unvalidated antibody issue. Labome, a biotech company based in New Jersey, has been developing the Validated Antibody Database (VAD) for the past 10 years through manual curation of formal publications by biomedical experts. As of July 2018, VAD contains 339,197 antibody application records for 61,608 validated antibodies, including 1,391 antibodies which are knockout-validated. The database covers 64,227 formal publications and includes antibodies against 7,624 human genes.  Each VAD record includes details about the antibody application (Western blot, immunohistochemistry, etc.), the cell type or tissue sample used, and the dilution, in addition to information about the antibody, such as its host species, clonality, and clone name. This database is freely available to the research community.

Plate Centrifuge

FastGene Plate Centrifuge is the most efficient, silent, and easy-to-use centrifuge for spinning down liquids in 96- and 384-well plates. Thanks to its compact size and light weight, it fits easily on a crowded lab bench. Centrifuging 96- and 384-well plates can be tricky if your lab is performing high-throughput assays for applications such as PCR, quantitative PCR, ELISA, and next-generation sequencing (NGS). Droplets or condensation on the sides of the wells can cause assay failure due to inadequate volumes or separation of reaction components from the reaction mixture, and liquid forced out of wells during centrifugation and into other wells will result in immediate contamination. FastGene uses an angle that allows finicky NGS applications to gain access to the bottom of the wells without disturbing the pellet. Its unique spinning parameters also keep the liquid in each well far away from the seal, with no way to contaminate other wells.

Diagnostics Platform

The Technology Partnership’s Puckdx point-of-care diagnostics platform uses a novel fluid-handling system designed to enable ready translation from bench to disposable. The platform has the flexibility to effectively and efficiently extract DNA and RNA from a range of samples. This flexibility, combined with its ultrafast thermal cycler module and multichannel optics detection system, makes the platform capable of generating rapid sample-to-answer results. Puckdx can run quantitative PCR amplification times of 7 min for 30 cycles. This will enable point-of-care diagnostics providers to quickly convert specific biological assays into a market-ready system capable of producing a result in less than a quarter of an hour. 

RNase-, DNase-, and Human DNA-Free Containers

For the rigorous requirements of molecular biology, genomic, and forensic research facilities, Thermo Fisher Scientific’s Sterilin universal containers are lot tested and certified to be free from RNase, DNase, human DNA, and pyrogens. They are aseptically produced under controlled, automated cleanroom conditions, and are designed for the safe handling of valuable or potentially hazardous samples, reducing the potential for contamination that could adversely affect analytical results. These clear containers are manufactured with robust polypropylene and deliver excellent, leak-free performance. They can accommodate a convenient working sample volume of up to 25 mL, ideal for a broad range of general laboratory applications, from sample handling to centrifugation. They are also ergonomically constructed, featuring a self-standing conical base and a half-turn Quickstart cap for easier handling. Each container has a unique lot number for traceability.

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