Mapping genotype to phenotype

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Science  02 Nov 2018:
Vol. 362, Issue 6414, pp. 555-556
DOI: 10.1126/science.362.6414.555-d

Identifying genetic factors that contribute to trait variation among species is difficult. Weiss et al. developed a method for unbiased genome-scale mapping of trait differences and used it to work out how a range of yeast species evolved. When crossed, these yeast species produce sterile offspring, preventing the use of traditional genetic-mapping techniques. However, by reciprocal hemizygosity analysis via sequencing (RH-seq), alleles that contributed to differences in thermotolerance were spotted that diverged between two species. Surprisingly, these turned out to map to essential housekeeping genes. Thus, without taking a candidate locus approach, RH-seq allowed identification of genes responsible for traits that differ between species.

Nat. Genet. 50, 1501 (2018).

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