MOLECULAR IMAGING

Revealing chromosome features

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Science  09 Nov 2018:
Vol. 362, Issue 6415, pp. 652-653
DOI: 10.1126/science.362.6415.652-g

Cryo–electron tomography (cryo-ET) is used to visualize cellular structures in the native environment without chemical fixation and dye labeling. Cai et al. used cryo-ET on both interphase and mitotic fission yeast cells to explore some interesting features of chromosome organization. Nucleosomes in situ do not appear to resemble the canonical conformation obtained by crystallography in vitro. In vivo, it appears that nucleosomes are partially unwrapped. Moreover, nucleosomes cluster irregularly, with the clusters being more condensed and less dynamic in mitotic cells. Yet, the condensation in mitotic chromosomes is uneven, and there are loosely packed regions where, possibly, mitotic transcription occurs.

Proc. Natl. Acad. Sci. U.S.A. 115, 10977 (2018).

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