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BALANTIDIA FROM PIGS AND GUINEA-PIGS: THEIR VIABILITY, CYST PRODUCTION AND CULTIVATION

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Science  22 Jul 1927:
Vol. 66, Issue 1699, pp. 89-91
DOI: 10.1126/science.66.1699.89

Abstract

It has been shown that trophozoites of balantidia from the pig are normal in appearance and reactions when the medium is cooled to room temperature. They may live at room temperature for ten days. Trophozoites that infect the pig may pass through the stomach of the guinea-pig and reach the cecum, where they are normal after eighteen hours. Trophozoites are frequently passed in feces by the pig but cyst production is irregular and determined by unknown factors as in monkeys, guinea-pigs and man. The fluid of pig feces from the cecum is slightly hypertonic to blood serum. The method of Barret and Yarbrough for the cultivation of Balantidium coli has been found practicable for Balantidium from the pig and guinea-pig. The addition of rice starch improves the medium and Ringer's solution without dextrose was found more suitable than 0.5 per cent. NaCl solution. Loeffler's beef blood serum may be substituted for human serum or horse serum. H2S appears non-toxic to Balantidium from the pig when passed into culture tubes.