ArticlesScientific Apparatus and Laboratory Methods

A SENSITIVE COLOR REACTION FOR THE DETECTION OF URACIL AND CYTOSINE

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Science  23 Jul 1943:
Vol. 98, Issue 2534, pp. 90-92
DOI: 10.1126/science.98.2534.90

Abstract

(1) Uracil and cytosine are both transformed quantitatively into 5,5-dichloroxyhydrouracil by interaction with superoxol and concentrated hydrochloric acid.

(2) This 5,5-dichloroxyhydrouracil reacts with barium hydroxide immediately at ordinary temperature giving the purple colored barium salt of dialuric acid.

(3) Both uracil and cytosine can be detected successfully in minute quantities by application of the technique discussed in this paper.