Supplemental Data


Abstract
Full Text
Regulation of Cutaneous Malignancy by namename T Cells
Michael Girardi, David E. Oppenheim, Carrie R. Steele, Julia M. Lewis, Earl Glusac, Renata Filler, Paul Hobby, Brian Sutton, Robert E. Tigelaar, Adrian C. Hayday

Supplementary Material

Supplemental Figure 1. (A) The percent sequence identity among Rae-1 isoforms. (B) Schematic representation of predicted Rae-1 structure domains. Four completely conserved N-linked glycosylation sites are shown in the name1 and name2 domains; the STP region is predicted to be heavily O-glycosylated.


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Supplemental Figure 2. (A) Surface representations of (left) Rae-1 modeled on ZAG; (center) the complex between MICA (below) and NKG2d (above in ribbon); and (right) MHC H2Db with bound peptide in green and name2-microglobulin in purple. All three structures are viewed in the same orientation with helix name1 in yellow and name2 in orange. Rae-1 has a stalk region (not shown), which is expected to be part extended and part helical and heavily O-glycosylated in addition to the GPI linkage. (B) Orthogonal views of Rae-1name and MICA, showing the similar distribution of charged residues at the interface. Residues indicated in Rae-1name are D175, E189 (red), R103, and K110 (blue); in MICA, they are D65, D149, D163 (red), R74, and K81 (blue). Figure was generated by INSIGHT.


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Supplemental Figure 3. Expression of Rae-1 and H60 in cell lines. Cell lines are Pam2-12, a keratinocyte line; CMT93, a colorectal carcinoma from C57/BL6 strain; NIH3T3 immortalized embryonic fibroblasts; L-929, a fibroblast-like cell line; J558L, an induced B cell myeloma from Balb/C strain; three thymomas: 4G4, C58, and 34.1; and CTLL2, an IL-2-dependent T cell line. An asterisk denotes that CTLL2 expresses neither Rae-1 nor H60 and did not stain with NKG2d beads.


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