Research Article

Integrative Analysis of the Caenorhabditis elegans Genome by the modENCODE Project

See allHide authors and affiliations

Science  22 Dec 2010:
1196914
DOI: 10.1126/science.1196914

This article has a correction. Please see:

Abstract

We systematically generated large-scale data sets to improve genome annotation for the nematode Caenorhabditis elegans, a key model organism. These data sets include transcriptome profiling across a developmental time course, genome-wide identification of transcription factor–binding sites, and maps of chromatin organization. From this, we created more complete and accurate gene models, including alternative splice forms and candidate noncoding RNAs. We constructed hierarchical networks of transcription factor–binding and microRNA interactions and discovered chromosomal locations bound by an unusually large number of transcription factors. Different patterns of chromatin composition and histone modification were revealed between chromosome arms and centers, with similarly prominent differences between autosomes and the X chromosome. Integrating data types, we built statistical models relating chromatin, transcription factor binding, and gene expression. Overall, our analyses ascribed putative functions to most of the conserved genome.

  • The modENCODE Consortium is a group of NHGRI-funded investigators defining genomic elements in C. elegans and D. melanogaster.