Targeted Genome Editing Across Species Using ZFNs and TALENs

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Science  23 Jun 2011:
DOI: 10.1126/science.1207773


Evolutionary studies necessary to dissect diverse biological processes have been limited by the lack of reverse genetic approaches in most organisms with sequenced genomes. Here, we established a broadly applicable strategy using zinc finger and TALE nucleases for targeted disruption of endogenous genes and cis-acting regulatory elements in diverged nematode species.