Research Article

The Structure of the Eukaryotic Ribosome at 3.0 Å Resolution

+ See all authors and affiliations

Science  17 Nov 2011:
1212642
DOI: 10.1126/science.1212642

Abstract

Ribosomes translate genetic information encoded by mRNA into proteins. Many aspects of translation and its regulation are specific to eukaryotes whose ribosomes are much larger and intricate than their bacterial counterparts. Here, we report the crystal structure of the 80S ribosome from the yeast Saccharomyces cerevisiae at 3.0 Å resolution that includes nearly all ribosomal RNA bases and protein side chains as well as an additional protein Stm1. This atomic model reveals the architecture of eukaryote-specific elements and their interaction with the universally conserved core, and describes all eukaryote-specific bridges between the two ribosomal subunits. It forms the structural framework for the design and analysis of experiments that explore the eukaryotic translation apparatus and the evolutionary forces that shaped it.