Report

Mouse B-Type Lamins Are Required for Proper Organogenesis But Not by Embryonic Stem Cells

+ See all authors and affiliations

Science  24 Nov 2011:
1211222
DOI: 10.1126/science.1211222

Abstract

B-type lamins, the major components of the nuclear lamina, are believed to be essential for cell proliferation and survival. We found that mouse embryonic stem cells (ESCs) do not need any lamins for self-renewal and pluripotency. Although genome-wide lamin-B binding profiles correlate with reduced gene expression, such binding is not required for gene silencing in ESCs or trophectoderm cells. However, B-type lamins are required for proper organogenesis. Defects in spindle orientation in neural progenitor cells and migration of neurons are most likely the causes of brain disorganizations found in lamin-B null mice. Thus, our studies not only disprove several prevailing views of lamin-Bs but also establish the foundation for redefining the function of the nuclear lamina in the context of tissue building.