Supplementary Materials

Cilia at the Node of Mouse Embryos Sense Fluid Flow for Left-Right Determination via Pkd2

Satoko Yoshiba, Hidetaka Shiratori, Ivana Y. Kuo, Aiko Kawasumi, Kyosuke Shinohara, Shigenori Nonaka, Yasuko Asai, Genta Sasaki, Jose Antonio Belo, Hiroshi Sasaki, Junichi Nakai, Bernd Dworniczak, Barbara E. Ehrlich, Petra Pennekamp, Hiroshi Hamada

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S14
  • References

Images, Video, and Other Other Media

Movie S1
Motility of cilia in the node as visualized by the fluorescence of Pkd2::Venus.
Transgenic embryos that harbor Foxa2-hsp-Pkd2::Venus and express Pkd2::Venus protein in pit cells and crown cells were analyzed. Red arrowheads indicate rotating cilia. Note that motile cilia in the node rotate normally.
Movie S2
Motility of cilia in node crown cells at the presomite stage.
Transgenic embryos that harbor NDE-hsp-Pkd2::Venus and express Pkd2::Venus protein in crown cells were analyzed at the presomite stage. Fluorescence of Pkd2::Venus was monitored for 10 seconds. Red arrowheads indicate rotating cilia, while blue arrowheads indicate immotile cilia. Note that most of the cilia are immotile.
Movie S3
Motility of cilia in node crown cells at the three-somite stage.
Transgenic embryos that harbor NDE-hsp-Pkd2::Venus and express Pkd2::Venus protein in crown cells were analyzed at the three-somite stage. Fluorescence of Pkd2::Venus was monitored for 10 seconds. Red arrowheads indicate rotating cilia, while blue arrowheads indicate immotile cilia.