Supplementary Materials

The Bacteriophage T7 Virion Undergoes Extensive Structural Remodeling During Infection

Bo Hu, William Margolin, Ian J. Molineux, Jun Liu

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S11
  • Table S1
  • Full References

Images, Video, and Other Other Media

Movie S1
Tomographic views of a T7-infected minicell. The T7 particle highlighted with a red arrow is that shown in Fig. 1A. White arrows indicate the two fiducial gold markers that in some frames result in the appearance of density across the cell envelope.
Movie S2
Dual axis tomography confirmed multiple conformations of T7 particles on an infected E. coli minicell. Two single-axis tomograms from the same minicell infected by T7 are shown (panels a, b). A dual axis tomogram (panel c) combines the two single axis tomograms, minimizing the artifacts derived from the "missing wedge" of tomography and improving resolution in the specimen plane. Cellular features, including the outer membrane, cell wall and inner membrane of the infected minicell are presented with better detail. In particular, the dual axis tomogram verifies the extended tail, which formed a channel between the two cell membranes.
Movie S3
Multiple conformations of T7 on the outer membrane. Fibers are bound to the outer membrane, followed by engagement of the tail, formation of the extended tail, and the empty capsid after DNA ejection. Note how the capsid and the tail move towards the outer membrane before the extended tail is formed.
Movie S4
The extended tail. Classification on the region including the extended tail reveals multiple conformations, suggesting that it is flexible
Movie S5
Animation of T7 infection. The key intermediate structures were determined in this work by cryo-ET. The transition between states was animated using UCSF Chimera.