Caffeoyl Shikimate Esterase (CSE) Is an Enzyme in the Lignin Biosynthetic Pathway

See allHide authors and affiliations

Science  15 Aug 2013:
DOI: 10.1126/science.1241602

You are currently viewing the abstract.

View Full Text


Lignin is a major component of plant secondary cell walls. Here, we describe caffeoyl shikimate esterase (CSE) as an enzyme central to the lignin biosynthetic pathway. Arabidopsis thaliana cse mutants deposit less lignin, and the remaining lignin is enriched in H units. Phenolic metabolite profiling identified accumulation of the lignin pathway intermediate caffeoyl shikimate in cse mutants compared to wild type, suggesting this as a substrate for CSE. Accordingly, recombinant CSE hydrolyzed caffeoyl shikimate into caffeate. Associated with the changes in lignin, the conversion of cellulose to glucose of cse mutants increased up to fourfold compared to wild type upon saccharification without pretreatment. Collectively, these data necessitate revision of currently accepted models of the lignin biosynthetic pathway.

View Full Text