Report

Molecular Editing of Cellular Responses by the High-Affinity Receptor for IgE

+ See all authors and affiliations

Science  06 Feb 2014:
1246976
DOI: 10.1126/science.1246976

You are currently viewing the abstract.

View Full Text

Abstract

Cellular responses elicited by cell surface receptors differ depending on stimulus strength. We investigated how the high-affinity receptor for IgE modulates the response of mast cells to a high- or low-affinity stimulus. Although both high- and low-affinity stimuli elicited similar receptor phosphorylation; receptor cluster size, mobility, distribution, and the cells’ effector responses differed. Low-affinity stimulation increased receptor association with the Src family kinase Fgr, and shifted signals from the adapter LAT1 to the related adapter LAT2. LAT1-dependent calcium signals required for mast cell degranulation were dampened, but the role of LAT2 in chemokine production was enhanced altering immune cell recruitment at the site of inflammation. The findings uncover how receptor discrimination of stimulus strength can be interpreted into distinct in vivo outcomes.

View Full Text