Research Article

The complete structure of the 55S mammalian mitochondrial ribosome

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Science  02 Apr 2015:
DOI: 10.1126/science.aaa3872

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Mammalian mitochondrial ribosomes synthesize mitochondrially encoded membrane proteins that are critical for mitochondrial function. Here we present the complete atomic structure of the porcine 55S mitoribosome at 3.8 Å resolution by cryo-electron microscopy and chemical crosslinking/mass spectrometry. The structure of the 28S subunit in the complex was resolved at 3.6 Å resolution by focused alignment, which allowed building of a detailed atomic structure including all of its 15 mitoribosomal-specific proteins. The structure reveals the intersubunit contacts in the 55S mitoribosome, the molecular architecture of the mitoribosomal mRNA binding channel and its interaction with tRNAs, and provides insight into the highly specialized mechanism of mRNA recruitment to the 28S subunit. Furthermore, the structure contributes to a mechanistic understanding of aminoglycoside ototoxicity.

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