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De novo synaptogenesis induced by GABA in the developing mouse cortex

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Science  11 Aug 2016:
aaf5206
DOI: 10.1126/science.aaf5206

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Abstract

Dendrites of cortical pyramidal neurons contain intermingled excitatory and inhibitory synapses. Here we study the local mechanisms that regulate the formation and distribution of synapses. We show that local γ-aminobutyric acid (GABA) release on dendrites of mouse cortical layer 2/3 pyramidal neurons can induce gephyrin puncta and dendritic spine formation via GABAA receptor activation and voltage-gated calcium channels during early postnatal development. Furthermore, the newly formed inhibitory and excitatory synaptic structures rapidly gain functions. Bidirectional manipulation of GABA release from somatostatin-positive interneurons increased and decreased the number of gephyrin puncta and dendritic spines, respectively. These results highlight a non-canonical function of GABA as a local synaptogenic element shaping the early establishment of neuronal circuitry in mouse cortex.

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