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Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9

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Science  10 Aug 2017:
eaan4187
DOI: 10.1126/science.aan4187

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  • RE: Pigs and xenotransplantation-related zoonotic risks
    • Giovanni Di Guardo, Associate Professor, University of Teramo, Faculty of Veterinary Medicine

    I have read with great interest the article by Dr Dong and coworkers (1), the results of which appear to be of great importance in relation to the control of the zoonotic risk posed by porcine endogenous retroviruses (PERVs) when dealing with swine organs and tissues used in xenotransplantation medical procedures.
    Beside the potential zoonotic hazard of PERVs, caution should be also taken with reference to hepatitis E virus (HEV), another pathogen of zoonotic concern, which may infect humans following either direct contact or ingestion of pork meat and/or pork meat-based products (2).
    In this respect, similarly to what successfully achieved with PERVs (1), it would be interesting to assess whether CRISPR-based technology may provide a valuable tool also for inactivating HEV, thus mitigating the zoonotic risk posed by this viral pathogen in xenotransplantation procedures involving biological materials derived from pigs.

    References

    1) Dong N., et al. (2017) - Science, August 10.

    2) Pavio N., et al. (2015) - Curr Opinion Virol 10:34-41.

    Competing Interests: None declared.
  • RE: Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9

    seems that the USA feed ban for ruminant protein is still a serious problem, so there seems to still be a risk factor for pigs and Transmissible Spongiform Encephalopathy TSE prion disease. now with the updated science showing that pigs are susceptible to the Chronic Wasting Disease TSE Prion ORALLY, and cwd running rampant in the USA, any use of porcine organs should be tested for the CWD TSE Prion...

    Research Project: TRANSMISSION, DIFFERENTIATION, AND PATHOBIOLOGY OF TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES

    Location: Virus and Prion Research

    Title: Disease-associated prion protein detected in lymphoid tissues from pigs challenged with the agent of chronic wasting disease

    Author item Moore, Sarah item Kunkle, Robert item Kondru, Naveen item Manne, Sireesha item Smith, Jodi item Kanthasamy, Anumantha item West Greenlee, M item Greenlee, Justin

    Submitted to: Prion Publication Type: Abstract Only Publication Acceptance Date: 3/15/2017 Publication Date: N/A Citation: N/A Interpretive Summary:

    Technical Abstract: Aims: Chronic wasting disease (CWD) is a naturally-occurring, fatal neurodegenerative disease of cervids. We previously demonstrated that disease-associated prion protein (PrPSc) can be detected in the brain and retina from pigs challenged intracranially or orally with the CWD agent. In that study, neurological signs consistent with prion disease were observed only in one pig: an intracranially challenged pig that was eutha...

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    Competing Interests: None declared.