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Pathogen blockade of TAK1 triggers caspase-8–dependent cleavage of gasdermin D and cell death

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Science  25 Oct 2018:
eaau2818
DOI: 10.1126/science.aau2818

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Abstract

Limited proteolysis of gasdermin D (GSDMD) generates an N-terminal pore-forming fragment, which controls pyroptosis in macrophages. GSDMD is processed via inflammasome-activated caspase-1 or -11. It is currently unknown whether macrophage GSDMD can be processed by other mechanisms. Here, we describe an additional pathway controlling GSDMD processing. The inhibition of TAK1 or IKK kinases by the Yersinia effector protein YopJ elicits RIPK1- and caspase-8–dependent cleavage of GSDMD, which subsequently results in cell death. GSDMD processing also contributes to the NLRP3 inflammasome–dependent release of interleukin-1β (IL-1β). Thus, caspase-8 acts as a regulator of GSDMD-driven cell death. Furthermore, this study establishes the importance of TAK1 and IKK activity in the control of GSDMD cleavage and cytotoxicity.

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