Supporting Online Material


Cellular and Subcellular Structure of Neoproterozoic Animal Embryos
James W. Hagadorn, Shuhai Xiao, Philip C. J. Donoghue, Stefan Bengtson, Neil J. Gostling, Maria Pawlowska, Elizabeth C. Raff, Rudolf A. Raff, F. Rudolf Turner, Yin Chongyu, Chuanming Zhou, Xunlai Yuan, Matthew B. McFeely, Marco Stampanoni, Kenneth H. Nealson

Supporting Online Material

This supplement contains:
Materials and Methods
SOM Text
Figs. S1 to S3
Movies S1 to S7

This file is in Adobe Acrobat PDF format.

Movie S1
Animation of serial x-ray attenuation images through 7-celled embryo in Fig. 2D (specimen MPLa). Image sizes and contrast spectra have been reduced in all movies to facilitate rapid download. Lighter greyscale values represent greater x-ray attenuation.

Movie S2
Animation of serial x-ray attenuation images through 2-celled embryo in Fig. 3A (specimen MPKxiv). Lighter greyscale values represent greater x-ray attenuation.

Movie S3
Animation of serial x-ray attenuation images through 4-celled embryo in Fig. 3E (specimen DOU-8). Darker greyscale values represent greater x-ray attenuation.

Movie S4
Animation of volume rendered cells and reniform subcellular structures in Fig 3E (specimen DOU-8). Two of the cells are rendered transparent and dislocated from the embryo to facilitate visualization.

Movie S5
Animation of serial x-ray attenuation images through 16-celled embryo in Fig. 1B (specimen DOU-10). Darker greyscale values represent greater x-ray attenuation. Specimen is ~820 m at its maximum diameter.

Movie S6
Animation sequence of x-ray attenuation images through 32-celled embryo (specimen DOU- 32). Darker greyscale values represent greater x-ray attenuation. Specimen is ~610 m at its maximum diameter.

Movie S7
Animation sequence of x-ray attenuation through a many-celled (~250 < n < 1250 cells) Megaclonophycus embryo (specimen PhilDii). Grey region in left-center of embryo reflects ovoidal cells where cell boundares are sutured together and/or where isopachous cements lining cell boundaries are compositionally less distinct from cell interiors. Lighter greyscale values represent greater x-ray attenuation. Specimen is 590 m at its maximum diameter.

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