Supporting Online Material


ATP Release Guides Neutrophil Chemotaxis via P2Y2 and A3 Receptors
Yu Chen, Ross Corriden, Yoshiaki Inoue, Linda Yip, Naoyuki Hashiguchi, Annelies Zinkernagel, Victor Nizet, Paul A. Insel, Wolfgang G. Junger

Supporting Online Material

This supplement contains:
Materials and Methods
Figs. S1 to S3
Table S1
References
Movies S1 to S10

This file is in Adobe Acrobat PDF format.

Video 1

Video 2
ATP release from human neutrophils in an fMLP gradient

Video 3
Negative control for ATP release assay (without glucose 6-phosphate dehydrogenase or NADP)

Video 4
Video 5
Neutrophils chemotaxis in the absence or presence of apyrase

Video 6
Neutrophils chemotaxis in the presence of 100 μM ATPγS

Video 7
Video 8
Neutrophils chemotaxis in the absence or presence of 10 μM MRS 1191

Video 9
Chemotaxis of HL60 cells expressing A3-EGFP fusion protein

Video 10
Confocal image sequence of HL60 cell expressing A3-EGFP fusion protein

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