Supporting Online Material

Subdiffraction Multicolor Imaging of the Nuclear Periphery with 3D Structured Illumination Microscopy
Lothar Schermelleh, Peter M. Carlton, Sebastian Haase, Lin Shao, Lukman Winoto, Peter Kner, Brian Burke, M. Cristina Cardoso, David A. Agard, Mats G. L. Gustafsson, Heinrich Leonhardt, John W. Sedat

Supporting Online Material

This supplement contains:
Materials and Methods
Figs. S1 to S6
References and Notes

This file is in Adobe Acrobat PDF format.

Other Supporting Online Material for this manuscript includes the following: available at
Movies S1 and S2

Movie s1
Three-dimensional view of the nucleus. 3dSIM imaging of nuclear pores (green) and DAPI staining (magenta) in a mouse C2C12 nucleus. The first part shows the slicing through the image stack; the second part shows the rotation of maximum-intensity projections around the x-axis. Scale bar, 5 μm.

Movie s1
3D volume rendering of a prophase nucleus imaged with 3dSIM, corresponding to Fig. 4. C2C12 cell is immunostained with antibodies against Lamin B and counterstained with DAPI (red). The volume rendering was performed with Volocity 4 software (Improvision).

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