Supporting Online Material


Synchronous Hyperactivity and Intercellular Calcium Waves in Astrocytes in Alzheimer Mice
Kishore V. Kuchibhotla, Carli R. Lattarulo, Bradley T. Hyman, Brian J. Bacskai

Supporting Online Material

This supplement contains:
Materials and Methods
Figs. S1 to S6
References

This file is in Adobe Acrobat PDF format.

Other Supporting Online Material for this manuscript includes the following: (available at www.sciencemag.org/cgi/content/full/323/5918/1211/DC1)
Movies S1 and S2

Movie s1
This movie shows a 10-minute time course of astrocytic spontaneous calcium activity in Layer I of the neocortex of a non-transgenic mouse. The cells in this image are loaded with the calcium sensitive dye, Oregon-Green BAPTA-1 AM, and the astrocyte specific marker, SR-101; the movie includes only the green channel to show the calcium activity. The green channel has not been contrast-adjusted but has been aligned in the X-Y dimensions. The field of view is ~300 μm and the scan rate is 0.33 Hz. A red circle denotes an astrocyte calcium transient. Note the relatively rare number of spontaneous transients that are observed in the aged, non-transgenic cortex.

Movie s2
This movie shows a 10-minute time course of astrocytic spontaneous calcium activity in Layer I of the neocortex of an APP/PS1 transgenic mouse with cortical plaques. The cells in this image are loaded with the calcium sensitive dye, Oregon-Green BAPTA-1 AM, and the astrocyte specific marker, SR-101; the movie includes the green channel to show the calcium activity. The green-channel has not been contrast-adjusted but has been aligned in the X-Y dimensions. Amyloid plaques stained with Methoxy-XO4 are overlaid on this image to show their location in the field. The field of view is ~300 μm and the scan rate is 0.33 Hz. A red circle denotes an astrocyte calcium transient. Compared to the non-transgenic from Movie S1, transgenic mice with cortical plaques exhibit increased spontaneous calcium transients that are occasionally synchronous in time.

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