Supporting Online Material

Meningococcal Type IV Pili Recruit the Polarity Complex to Cross the Brain Endothelium
Mathieu Coureuil, Guillain Mikaty, Florence Miller, Hervé Lécuyer, Christine Bernard, Sandrine Bourdoulous, Guillaume Duménil, René-Marc Mège, Babette B. Weksler, Ignacio A. Romero, Pierre Olivier Couraud, Xavier Nassif

Supporting Online Material

This supplement contains:
Materials and Methods
Figs. S1 to S7

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Other Supporting Online Material for this manuscript includes the following: available at
Movies S1 and S2

Movie s1
Formation of the N. meningitidis microcolony onto the apical surface of endothelial cells. Endothelial cells grown on a glass slide were placed under a fluorescence microscope at 37°C and 5% CO2. Bacteria growing on the top of the cells were followed by the use of a time-lapse video microscopy. Real-time duration is 200 min.

Movie s2
3D reconstruction from a stack of 0.12μm interval images, acquired using a Leica SP5 confocal microscope. TEA1/31 bound VE-cadherin (green) is relocated from cell-cell junctions underneath the N. meningitidis colony.

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