Supporting Online Material

Activation of Rho GTPases by DOCK Exchange Factors Is Mediated by a Nucleotide Sensor
Jing Yang, Ziguo Zhang, S. Mark Roe, Christopher J. Marshall, David Barford

Supporting Online Material

This supplement contains:
Materials and Methods
Figs. S1 to S8
Table S1

This file is in Adobe Acrobat PDF format.

Other Supporting Online Material for this manuscript includes the following: available at
Movies S1 and S2

Movie s1
Movie S1 illustrates the conformational changes of Cdc42 in transition from the GDP-bound state (10) to the nucleotide-free state as observed in the DOCK9DHR2-Cdc42 complex. GDP/Mg2+ shown is as bound to the Cdc42-GDP/Mg2+ complex. Switch1 (red) undergoes a large conformational change, whereas the P-loop (blue) and switch2 (magenta) remain essentially unchanged.

Movie s2
Movie S2 illustrates the guanine nucleotide exchange cycle. In the initial 10 frames, the structure of the DOCK9DHR2-Cdc42-GDP complex is shown. This is morphed to the nucleotide free-DOCK9DHR2-Cdc42 complex over 30 frames.

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