Supporting Online Material

Planarian Hh Signaling Regulates Regeneration Polarity and Links Hh Pathway Evolution to Cilia
Jochen C. Rink, Kyle A. Gurley, Sarah A. Elliott, Alejandro Sánchez Alvarado

Supporting Online Material

This supplement contains:
Materials and Methods
Figs. S1 to S23

This file is in Adobe Acrobat PDF format.

Other Supporting Online Material for this manuscript includes the following:
Movies S1 to S3

Movie s1
Control RNAi-treated animals. Behavior of normal body fragments after regeneration. Animals were fed dsRNA against the C. elegans gene unc-22. Wild type animals were amputated pre- and post-pharyngeally and the resulting fragments allowed to regenerate. Heads and tails regenerated normally in the appropriate anterior and posterior amputation planes. When exposed to light, the animals respond by gliding away from the source using the cilia that covers their ventral surface.

Movie s2
Activation of the Hh signaling pathway. ptc(RNAi) trunk fragments regenerate tails at both anterior and posterior amputation planes. The worms are still alive, but both ends attempt to move toward the center of the animal.

Movie s3
Inhibition of fused. After pre- and post-pharyngeal amputation, fused(RNAi) trunk fragments regenerate heads and tails normally and do not display phenotypes associated with defects in Hh signaling. However, the animals display motility defects ("inchworming" rather than gliding), indicating a role of this molecule in normal cilia functioning.

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