Supporting Online Material

Requirement of Prorenin Receptor and Vacuolar H+-ATPaseā€”Mediated Acidification for Wnt Signaling
Cristina-Maria Cruciat, Bisei Ohkawara, Sergio P. Acebron, Emil Karaulanov, Carmen Reinhard, Dierk Ingelfinger, Michael Boutros, Christof Niehrs

Supporting Online Material

This supplement contains:
Materials and Methods
Figs. S1 to S13
Table S1

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Other Supporting Online Material for this manuscript includes the following:

Movie s1
Time laps live cell imaging in HEK293T cells transfected with Ra-LRP6 (green) and membrane-anchored RFP (red). Cells were treated with Wnt3a conditioned medium for 5 min before acquisition of images. Ra-LRP6 was excited at 470 nm, to monitor the protein in acidic compartments. Acquisition time is indicated in the movie.

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