Supporting Online Material


Dynamics of Dpp Signaling and Proliferation Control
O. Wartlick, P. Mumcu, A. Kicheva, T. Bittig, C. Seum, F. Jülicher, M. González-Gaitán

Supporting Online Material

This supplement contains:
Materials and Methods
SOM Text
Figs. S1 to S53
Tables S1 to S5
References

This file is in Adobe Acrobat PDF format.

Other Supporting Online Material for this manuscript includes the following:

Movie s1
z-projection of dad-nRFP expressing wing disc (t ~ 96 hours) imaged in vivo at a frame rate of 3 minutes. In this movie the dad-nRFP intensity increases by about 9% per hour.

Movie s2
Tissue growth simulated with the temporal growth rule and parameter set I (wing). Time t is given in hours (h) after start of the simulation. Several representations (A/P compartments, Hh distribution, Dpp transcriptional activation, Dpp distribution, Cell cycle states) of the simulated tissue are shown in sequence to illustrate the setup of the simulation (for details see SOM - Simulation).
A/P compartments: blue, anterior; red, posterior cells; white lines, bonds corresponding to compartment boundaries;
Hh distribution: cyan; note that the color scale is rescaled in each frame, so that the brightest color is always assigned to the cell with the highest number of molecules;
Dpp transcriptional activation: cells which produce Dpp molecules in response to sufficiently high Hh levels are marked with a white dot;
Dpp distribution: green; note that the color scale is rescaled in each frame, so that the brightest color is always assigned to the cell with the highest number of molecules; Cell cycle states: red, M-phase; blue, constant part of interphase; black, checkpoint phase.

Movie s3
Tissue growth simulated with the temporal growth rule and parameter set I (wing). For details of the growth rule see SOM SI4. Time t is given in hours (h) after start of the simulation. The color code shows the cell cycle state (red: M-phase, blue: constant part of interphase, black: checkpoint phase).

Movie s4
Tissue growth simulated with the spatial growth rule and parameter set I (wing). For details of the growth rule see SOM SI4. Time t is given in hours (h) after start of the simulation. The color code shows the cell cycle state (red: M-phase, blue: constant part of interphase, black: checkpoint phase). Note the waves of proliferation caused by local instabilities. Growth generated with this rule does not stop.

Movie s5
Tissue growth simulated with a spatial growth rule with time averaging and parameter set I (wing). For details of the growth rule see SOM SI4. Time t is given in hours (h) after start of the simulation. The color code shows the cell cycle state (red: M-phase, blue: constant part of interphase, black: checkpoint phase). Note that proliferation is reduced in the source and the distal edge of the gradient.

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