Supporting Online Material

Electrical Spiking in Escherichia coli Probed with a Fluorescent Voltage-Indicating Protein

Joel M. Kralj, Daniel R. Hochbaum, Adam D. Douglass, Adam E. Cohen

Materials/Methods, Supporting Text, Tables, Figures, and/or References

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  • Materials and Methods
  • SOM Text
  • Figs. S1 to S11
  • Tables S1 to S6
  • References (18–33)
  • Movies

Images, Video, and Other Other Media

Movie S1
E. coli strain BW25113 expressing PROPS imaged under high redlight intensity (λ = 633 nm, I = 20 W/cm2). Cells were in minimal medium (pH 8) at roomtemperature. Movie is sped up by 4x.
Movie S2
E. coli strain BW25113 expressing PROPS imaged under low redlight intensity (λ = 633 nm, I = 1 W/cm2). Cells were in minimal medium (pH 7) at roomtemperature. Movie is sped up by 8x.
Movie S3
E. coli strain JY29 expressing PROPS adhered to a coverslip by asticky flagellum. Cells were in minimal medium (pH 8.5). Movie is slowed down by 3x.
Movie S4
E. coli strain BW25113 expressing PROPS exposed to violet light (λ= 407 nm, I = 100 W/cm2) while imaging PROPS (λ = 633 nm, I = 20 W/cm2). Cells were inminimal medium (pH 7) at room temperature. Movie is sped up by 6x.
Movie S5
E. coli strain BW25113 expressing PROPS during oxygen removalwith the chemical system Oxyrase. Oxyrase was dissolved in minimal medium (pH 7) accordingto the manufacturer’s directions. Imaging at λ = 633 nm, I = 20 W/cm2. Movie is sped up by 6x.
Movie S6
E. coli strain BW25113 expressing PROPS during azide addition (10mM). Imaging at λ = 633 nm, I = 20 W/cm2. Movie is sped up by 6x.
Movie S7
E. coli strain BW25113 expressing PROPS during CCCP addition (50μg/mL). Imaging at λ = 633 nm, I = 20 W/cm2. Movie is sped up by 6x.