Supporting Online Material

High-Speed Atomic Force Microscopy Reveals Rotary Catalysis of Rotorless F1-ATPase

Takayuki Uchihashi, Ryota Iino, Toshio Ando, Hiroyuki Noji

Materials/Methods, Supporting Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S12
  • Table S1
  • References (26-28)

Images, Video, and Other Other Media

Movie S1
AFM movie of the C-terminal side of α3β3 without nucleotide. Scan area, 18 x 15 nm2; frame rate, 10 fps.
Movie S2
AFM movie of the N-terminal side of α3β3 without nucleotide. Scan area, 17 x 11 nm2; frame rate, 10 fps.
Movie S3
AFM movie of the C-terminal side of α3β3 in 2 µM ATP. Scan area, 17 x 13 nm2; frame rate, 12.5 fps.
Movie S4
AFM movie of the C-terminal side of α3β3 in 3 µM ATP. Scan area, 18 x 14 nm2; frame rate, 12.5 fps.
Movie S5
AFM movie of the C-terminal side of α3β3 in 4 µM ATP. Scan area, 17 x 11 nm2; frame rate, 12.5 fps.
Movie S6
AFM movie of the C-terminal side of α3β3 in 2 µM ATP. Scan area, 21 x 14 nm2; frame rate, 12.5 fps. The pixel with the highest (brightest) position in each image is indicated by the blue circle. The center used for calculating the rotational angle is indicated by the cross mark.
Movie S7
AFM movie of the C-terminal side of α3β3 in 2 µM ATP. Scan area, 21 x 14 nm2; frame rate, 12.5 fps. At 16 s, one β subunit was lost.
Movie S8
AFM movie of the C-terminal side of α3β3 in 2 µM ATP. Scan area, 25 x 14 nm2; frame rate, 12.5 fps. At 9 s, one α subunit was lost.