Supplementary Materials

Mechanical Control of Morphogenesis by Fat/Dachsous/Four-Jointed Planar Cell Polarity Pathway.

Floris Bosveld, Isabelle Bonnet, Boris Guirao, Sham Tlili, Zhimin Wang, Ambre Petitalot, Raphaël Marchand, Pierre-Luc Bardet, Philippe Marcq, François Graner, Yohanns Bellaïche

Materials/Methods, Supporting Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Supplementary Text
  • Figs. S1 to S20
  • References
Revised 3 May 2012: Several minor typographical errors and figure panel callouts were corrected throughout.
References (53) and (54) were added to the reference list.On page 27, the last sentence of the first
paragraph now reads, "We interpret this absence of correlation by stating that the junction tension does not
depend on its orientation." On page 36, the scale bar was restored to fig. S15. On page 36, genotype labels
were restored to the left of fig. S16.
The original version is still available here.

Images, Video, and Other Other Media

Movie S1
Drosophila pupa dorsal thorax tissue expressing E-Cad:GFP to label apical cell junctions, imaged by multi-position confocal microscopy (24 positions at a 5 min time resolution) between 11 and 35 hAPF. The boxed region, highlighting the scutellum, is magnified at the left. The positions of the macrochaetae and of the midline are indicated by white circles and by a black dotted line, respectively. Arrows indicate the A/P and M/L axes. For compression reasons, these images have half the resolution of original movie.
Movie S2
Tracking of cell dynamics in the scutellum and the posterior scutum. Note that, in order to show the full color code, the above still image is the last image of the movie. (A) Dorsal thorax tissue labeled with E-Cad:GFP and imaged by multi-position confocal microscopy at a 5 min time resolution between 11:30 and 27:30 hAPF. (B) Tracking of cell trajectories. (C) Tracking of cell divisions, apoptoses and rearrangements. Each cell is color coded according to the number of cell divisions that it undergoes during the movie: pinkfor cells having divided once; blue for cells having divided twice; green for the third wave of division. Links used to calculate cell rearrangement matrix (section 4.2) are shown as lines. Two cells which are in contactin an image, and not in the next one, correspond to a lost link, plotted as a yellow line. Two cells which get in contact in an image, and were not in contact in the previous one, correspond to a gained link, plotted as a red line. Links involving a four-fold vertex are shown in lighter colours. Yellows zones indicate an apoptosis or delamination (which we do not distinguish). Light blue cells indicate new cells that entered the field ofview. (C’) Blow up of the region delimited in C by a cyan square. Scale bars (A-C): 10 μm.
Movie S3
Local tissue flow, deformation rate and rotation rate measured on Movie S1. Left: flow velocity is represented as arrows. Middle: deformation rate is represented as ellipses; a direction of elongation is represented in red and contraction in blue. Right: rotation rate is represented by circle diameters; clockwise is represented in red and counter-clockwise in blue. For compression reasons, only one image every half hour is shown. Scale bars: 100 μm (black bars), 9×102 μm min1 (arrow, left), 2.4×103 min1 (bluebar, middle), 8×105 min1 (circle, right).
Movie S4
Scutellum labeled with D:GFP and Baz:mCherry during morphogenesis (11 to 26 hAPF). The positions of the macrochaetae and of the midline are indicated by yellow circles and by a cyan dashed line, respectively. Arrows indicate the A/P and M/L axes.
Movie S5
Representative movies of cell junction ablations with high (left) or low (right) D:GFP signal in the scutellum of pupae expressing D:GFP and Baz:mCherry.