Supplementary Materials

Differential Diffusivity of Nodal and Lefty Underlies a Reaction Diffusion Patterning System

Patrick Müller, Katherine W. Rogers, Ben M. Jordan, Joon S. Lee, Drew Robson, Sharad Ramanathan, Alexander F. Schier

Materials/Methods, Supporting Text, Tables, Figures, and/or References

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  • Texts S1 to S8
  • Tables S1 to S8
  • Figs. S1 to S26

Images, Video, and Other Other Media

Movie S1
Squint-Dendra2 clearance assay experiment. An embryo ubiquitously expressing Squint-Dendra2 was imaged immediately prior to photoconversion and every 10 min after photoconversion as described in Text S5. Imaging began between dome and 30% epiboly stages (˜5 hours post-fertilization). Left: red fluorescence from photoconverted Squint-Dendra2. Note the high-intensity extracellular fraction that appears immediately post-photoconversion and decays over time. Intracellular intensities also increase after photoconversion, but the majority of the protein appears to be localized extracellularly. In control experiments, we established that the focal plane stays constant and that slight changes in embryo diameter are likely due to tissue morphogenesis. Right: Alexa488-dextran was injected at the one-cell stage in order to label all cells. The Alexa488 signal was used to generate masks that define the extracellular space (fig. S13, Text S5). The average red extracellular intensity was determined in each image, and the time-dependent decay was fitted with an exponential function, yielding a half-life of 99 min for this embryo.