Supplementary Materials

Coagulation Factor X Activates Innate Immunity to Human Species C Adenovirus

Konstantin Doronin, Justin W. Flatt, Nelson C. Di Paolo, Reeti Khare, Oleksandr Kalyuzhniy, Mauro Acchione, John P. Sumida, Umeharu Ohto, Toshiyuki Shimizu, Sachiko Akashi-Takamura, Kensuke Miyake, James W. MacDonald, Theo K. Bammler, Richard P. Beyer, Frederico M. Farin, Phoebe L. Stewart, Dmitry M. Shayakhmetov

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S18
  • Tables S1 to S3
  • References (22ā€“29)

Images, Video, and Other Other Media

Movie S1
Cryo-EM visualization of the FX-HAdv5 complex. Section one of the movie shows the 38ƅ FX-HAdv5 cryo-EM structure3 with the HAdv5 capsid in blue and FX in red. Section two shows the same cryo-EM structure but with only the strongest FX density in the central depressions of each hexon trimer shown. Section three shows the new, higher resolution, FX-HAdv5 cryo-EM structure using a slightly modified color scheme with the HAdv5 penton base in gold and fiber in green.
Movie S2
Resolving adenovirus ā€” FX complex binding interface with MDFF. The movie starts with the best rigid body fit FX orientation (red ribbon) above the hexon (light blue ribbon). The new, higher resolution cryo-EM density of a small portion of the Ad5/FX structure is shown (solid gray fading to transparent gray). View and scale change to show the protein-protein interface between the FX-GLA domain and hexon. Side chains are shown for K10 of the FX-GLA domain along with E424 and T425 of hexon. Ca+2 ions (green) in the zymogenic FX model remain associated with the FX GLA domain during this simulation. The movement of atoms during 80ps of the MDFF trajectory is shown and looped through twice.