Supplementary Materials

Interstitial Dendritic Cell Guidance by Haptotactic Chemokine Gradients

Michele Weber, Robert Hauschild, Jan Schwarz, Christine Moussion, Ingrid de Vries, Daniel F. Legler, Sanjiv A. Luther, Tobias Bollenbach, Michael Sixt

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S10
  • References

Images, Video, and Other Other Media

Movie S1
Migration of TAMRA-labeled DCs (red) into lymphatic vessels (LYVE-1 staining, green) in ear explants visualized by confocal microscopy. Time-lapse recorded over 40 min (20 frames, 10 frames/sec) Scale bar: 100 µm.
Movie S2
Highlighting migration pattern of a TAMRA-labeled DC (grayscale, indicated by arrow) between two lymphatic vessels (indicated by the yellow lines) in ear explant visualized by confocal microscopy. Time-lapse recorded over 60 min (30 frames, 10 frames/sec). Scale bar: 50 µm.
Movie S3
Vector maps to visualize the local increase in CCL21 or CCL19 concentration. The averaged concentration as sensed by cells of the indicated diameter is shown for each chemokine staining as the grayscale image background together with the polarization (red arrows). Scale bar: 15 µm. LV indicates the position of the lymphatic vessel.
Movie S4
Migration of TAMRA-labeled DCs (red) into lymphatic vessels (LYVE-1 staining, green) in ear explants pre-treated with 0.6 μg/ml CCL21, as visualized by confocal microscopy. Time-lapse recorded over 60 min (30 frames, 10 frames/sec) Scale bar: 100 µm.