Supplementary Materials

Sex Differences in the Gut Microbiome Drive Hormone-Dependent Regulation of Autoimmunity

Janet G. M. Markle, Daniel N. Frank, Steven Mortin-Toth, Charles E. Robertson, Leah M. Feazel, Ulrike Rolle-Kampczyk, Martin von Bergen, Kathy D. McCoy, Andrew J. Macpherson, Jayne S. Danska

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S4
  • Tables S1, S5, and S6
  • Legends for tables S2 to S4
  • References
Table S2
FIA-MS metabolomics data. Serum levels of 145 metabolites, including acylcarnitines, glycerophospholipids, and sphingolipids, were measured by flow injection analysis mass spectroscopy (FIA-MS). Raw data from FIA-MS metabolomics profiling of germ free (GF) and specific pathogen free (SPF) NOD males and females, used to generate the principal component analysis (PCA) presented in Fig 1D. Raw data from FIA-MS profiling of microbiome transfer recipients. Serum concentrations of all metabolites are shown in μM. Nonparametric t-test p values, and fold change (FC), negative log p-values, and log (FC) values are shown for each pairwise comparison.
Table S3
LC-MS/MS metabolomics data. Serum levels of 38 metabolites, including
amino acids and biogenic amines, were measured by liquid chromatography (LC)-MS/MS. Raw data from LC-MS/MS metabolomics profiling of germ free (GF) and specific pathogen free (SPF) NOD males and females, used to generate the principal component analysis (PCA) presented in Fig 1D. Raw data from LC-MS/MS profiling of microbiome transfer recipients. Serum concentrations of all metabolites are shown in μM. Non-parametric t-test p values, and fold change (FC), negative log p-values, and log (FC) values are shown for each pairwise comparison.
Table S4
16S sequencing of the cecal microbiota of un-manipulated and microbiome
transplant recipient mice.
Cecal bacterial DNA was prepared from NOD female recipients of adult male biome (M→F) or adult female biome (F→F) aged 14 weeks, as well as recipients of the male biome aged 34 weeks (M→F 34wk) and control, un-manipulated males and females. Bacterial 16S rDNA sequencing was performed, sequences were assigned to genus-level taxa, and various pairwise comparisons of genera abundances between biological groups were performed. Raw data is displays for all genus-level taxonomic assignments. P values were generated by pair-wise comparisons, two-part statistic (35).