Supplementary Materials

A Localized Wnt Signal Orients Asymmetric Stem Cell Division in Vitro

Shukry J. Habib, Bi-Chang Chen, Feng-Chiao Tsai, Konstantinos Anastassiadis, Tobias Meyer, Eric Betzig, Roel Nusse

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

Download Supplement
  • Materials and Methods
  • Figs. S1 to S12
  • Captions for movies S1 to S10
  • References (3033)

Images, Video, and Other Other Media

Movie S1
Three-dimensional view of a dividing H2B-Venus ES contacting Wnt3a bead. Images were collected by Bessel beam plane illumination microscopy every 30s.
Movie S2
Three-dimensional view of a dividing H2B-Venus ES contacting Wnt5a bead. Images were collected by Bessel beam plane illumination microscopy every 30s.
Movie S3
A Nanog-Venus ES cell contacting Wnt3a bead. Images were captured every 20 minutes.
Movie S4
A Rex1-GFP ES cell contacting Wnt3a bead. Images were captured every 20 minutes.
Movie S5
A Sox2-GFP ES cell contacting Wnt3a bead. Images were captured every 20 minutes.
Movie S6
A Stella-GFP ES cell contacting Wnt3a bead. Images were captured every 20 minutes.
Movie S7
A Nanog-Venus ES cell contacting Wnt5a bead. Images were captured every 20 minutes.
Movie S8
A Rex1-GFP ES cell contacting Wnt5a bead. Images were captured every 20 minutes.
Movie S9
A Sox2-GFP ES cell contacting Wnt5a bead. Images were captured every 20 minutes.
Movie S10
A Stella-GFP ES cell contacting Wnt5a bead. Images were captured every 20 minutes.