Supplementary Materials

High-speed recording of neural spikes in awake mice and flies with a fluorescent voltage sensor

Yiyang Gong, Cheng Huang, Jin Zhong Li, Benjamin F. Grewe, Yanping Zhang, Stephan Eismann, Mark J. Schnitzer

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S9
  • Tables S1 and S2
  • References

Images, Video, and Other Other Media

Movie S1
Action potential dynamics in a visual cortical neuron of an awake mouse. A movie of the spike-triggered average fluorescence response (ΔF/F) before, during and after firing of an action potential, for the layer 2/3 visual cortical neuron of Fig. 3C as imaged using Ace2N-4AA-mNeonGreen in an awake mouse. The movie depicts a time interval of 6.5 ms and represents an average over 1,900 spikes, mutually aligned to the times of action potential peaks at the soma. The left two dendrites activate before the right two dendrites. The time increment between successive image frames is 0.25 ms, and the playback speed is 10 frames · s-1. Spatial scale bar: 40 μm.
Movie S2
Action potential dynamics of a neuron in the antenna lobe of an intact fly brain. A movie of the spike-triggered average fluorescence response (ΔF/F) before, during and after firing of an action potential, for the antenna lobe neuron of Fig. 4C, D as imaged using Ace2N- 2AA-mNeonGreen in an intact fly brain explant. The movie depicts a time interval of 9.25 ms and represents an average over 1,300 spikes, mutually aligned to the times of action potential peaks as recorded electrically at the soma (Fig. 4C). Depolarization started at about –1.0 ms in the soma (located in upper right of each image) and propagated right to left across the dendritic tree during a spike. The time increment between successive image frames is 0.25 ms. The playback speed is 10 frames · s-1. Spatial scale bar: 40 μm.