Supplementary materials

Basidiomycete yeasts in the cortex of ascomycete macrolichens

Toby Spribille, Veera Tuovinen, Philipp Resl, Dan Vanderpool, Heimo Wolinski, M. Catherine Aime, Kevin Schneider, Edith Stabentheiner, Merje Toome-Heller, Göran Thor, Helmut Mayrhofer, Hanna Johannesson, John P. McCutcheon

Materials/Methods, Supplementary Text, Tables, Figures, and/or References

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  • Materials and Methods
  • Figs. S1 to S16
  • Tables S1 to S12
  • Full Reference List
  • Captions for Movies S1 and S2
Table S6
Table S7

Images, Video, and Other Media

Movie S1
Animation of a ~ 15 μm 3D z-stack through the cortex region of a fluorescently labeled thallus of Bryoria tortuosa. Secondary metabolite (vulpinic acid) residues autofluoresce in the same channel as the CY3-labeled ascomycete (purple), while 6-FAM-labeled Cyphobasidiales (basidiomycete) yeasts are rendered in green. Algae are located below and out of view of the z-stack.
Movie S2
Animation of a ~100 μm 3D z-stack through a fluorescently hybridized thallus of Bryoria capillaris. Cyphobasidiales (basidiomycete) yeasts are rendered in green, lecanoromycete (ascomycete) filamentous fungi are rendered in purple, and algae (Trebouxia simplex) are rendered in red.