Table 1

Genomes that have been assembled from 11 pieces and successfully transplanted. Assembly 2-100, 1; assembly 101-200, 2; assembly 201-300, 3; assembly 301-400, 4; assembly 401-500, 5; assembly 501-600, 6; assembly 601-700, 7; assembly 701-799, 8; assembly 811-900, 9; assembly 901-1000, 10; assembly 1001-1104, 11. WM, watermarked assembly.

Genome assemblySynthetic fragmentsNatural fragments
Reconstituted natural genomeNone1–11
2/11 semisynthetic genome with one watermark5 WM, 101–4, 6–9, 11
8/11 semisynthetic genome without watermarks1–4, 6–8, 115, 9, 10
9/11 semisynthetic genome without watermarks1–4, 6–8, 10–115, 9
9/11 semisynthetic genome with three watermarks1, 2 WM, 3 WM, 4, 6, 7 WM, 8, 10–115, 9
10/11 semisynthetic genome with three watermarks1, 2 WM, 3 WM, 4, 5 WM, 6, 7 WM, 8, 10–119
11/11 synthetic genome, 811-820 correction of dnaA1, 2 WM, 3 WM, 4, 5 WM, 6, 7 WM, 8, 9–11None
11/11 synthetic genome, 811-900 correction of dnaA1, 2 WM, 3 WM, 4, 5 WM, 6, 7 WM, 8, 9–11None