Table 2

Intracellular radiolabeled 73AsO4 arsenate distribution. All major cellular subfractions contained radiolabel after cell-washing procedures. Small molecular weight (s.m.w.) metabolites potentially include arsenylated analogs of ATP, NADH, acetyl-CoA, and others (11). SE is shown.

Solvent (subcellular fraction)Cellular radiolabel recovered (percent of total)
Phenol (protein + s.m.w. metabolites)80.3 ± 1.7
Phenol:Chloroform (proteins + lipids)5.1 ± 4.1
Chloroform (lipids)1.5 ± 0.8
Final aqueous fraction (DNA/RNA)11.0 ± 0.1