Simplified Way to Cultivate Chick Kidney Cells and Maintain the Culture without Serum

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Science  25 Sep 1959:
Vol. 130, Issue 3378, pp. 793
DOI: 10.1126/science.130.3378.793


Chick kidney fragments were easily dispersed after incubation in trypsin solution for 1 hour at room temperature. The centrifuged cells were resuspended in Melnick's growth medium, diluted to 100 ml for each pair of kidneys, and seeded at 1 ml per tube. The cultures were maintained for 7 days or longer in the medium modified by replacing the serum with tryptose.

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