Virus Production by Subcultured Monkey-Kidney Cells

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Science  25 Mar 1960:
Vol. 131, Issue 3404, pp. 936
DOI: 10.1126/science.131.3404.936


Cultured monkey-kidney cells, like the rabbit cells previously studied, have a relatively high folic acid requirement for growth. Subcultures can be readily prepared if the medium is supplemented with a high level of the vitamin. The sensitivity of the resultant cells to poliovirus and the yields of virus obtainable with them are the same as with primary cultures.