Colloidal Silica as a Standard for Measuring Absolute Fluorescence Yield

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Science  27 May 1960:
Vol. 131, Issue 3413, pp. 1611-1612
DOI: 10.1126/science.131.3413.1611


The use of colloidal silica as a standard for fluorescence efficiency measurements is proposed. Its advantages over glycogen as a standard are availability, purity, and stability. Absolute fluorescence yields are reported for a number of stubstiances and compared with values in the literatUre.